High-Throughput Gene Knockin Service
SimpleGene Tech, where our leading high-throughput gene knockin technology platform, can construct drug target reporter cell lines within 3 weeks. This facilitates the provision of cell products and customized services for drug target validation, lead compound screening, and studies of the mechanism of action (MOA) in various stages of drug development.
Technical Advantages of SimpleGene Tech
1.High-Throughput Single-Site Integration the Hambor Cell Platform
(1)Combined with the pCargo vector for single-copy integration of the safe harbor site (Hambor).
(2)Pre-prepared Harbor mother cell lines with multiple cell backgrounds can express target genes in different cells.
(3)Compared to lentivirus and other random integrations, all cell have uniform integration sites and copy numbers, leading to more stable genetics and clearer phenotypes.
(4)Efficient screening method enable the acquisition of stable expressing cell lines within 2 weeks.
(5)The pCargo series vectors come with various expression elements, offering options for either constitutive or inducible expression.
2.High-Throughput Muiti Site Integration Using the Shotgun Cell Platfornn
(1)Combined with the pLMPB vector to achieve integration of multiple sites in the genome.
(2)High integration efficiency can ensure genome integration of target genes in any cell background.
(3)Compared with lentivirus and other random integrations, it is simpler, safer and more efficient to use.
(4)Efficient screening method, obtain stable high-expression cell lines in 2 weeks.
(5)The pLMPB series vectors carry a variety of expression components and can be selected for continuous expression or induced expression.
(6)The inserted gene can be reversibly removed from the cell to confirm that the phenotype originates from the target gene.
Services at SimpleGene Tech
1.Pre-fabricated pCargo vectors
2.Ready-to-use pharmaceutical target reporter cell lines
3.Single-site integration in Hambor cell lines
4.Multi-site integration with the shotgun stable transformation service
At SimpleGene Tech, we’re committed to delivering unparalleled solutions that streamline and accelerate your research and development endeavors in the field of biotechnology. Partner with us to unlock the full potential of your scientific aspirations.