KO Cell Lysis
The KO cell pool was obtained by knocking out the gene in HEK293T cells using RNP method, and the KO cell lysate was prepared by lysis.
Quality Control
Verification by Sanger sequencing: knock-out efficiency >80%.
Proteomics Analysis Results of WT Cells
The gene is expressed in HEK293T cells.
Necessity Analysis of Target Gene in Target Cells
The gene is classified as non-essential in HEK293T cells.
SimpleGene Tech provide 100ug cell precipitation for WB, MS detection and other related experiments.